NANOMEDICINE

NAnoparticle DRUG LOAD & UNLOADING MEASUREMENT

Accurate nanoparticle characterisation for nanomedicine development can be expensive, slow and inaccurate. Oxford HighQ has developed an instrument that allows rapid and consistent measurement of drug load across a nanoparticle population (both surface coating or internal drug loading) for targeted drug delivery through measurement of refractive index, providing a resolution of a few percent for particles from about 50 nm to 500 nm in size.

Our nanoparticle analyser solves the problem of measuring nanoparticle size and drug load and unloading using a bench top analytical instrument that is fast, accurate, easy to use and affordable to the general research community.

We have successfully demonstrated our ability to accurately measure the drug offloading profile of cetylpyridinium chloride (CPC) from mesoporous silica nanoparticles. The figure opposite demonstrates the mean drug offloading as a function of time using a rolling average window of 350 seconds. A steady decrease followed by a sharp release is observed with full drug unloading seen after 40 minutes. For full results and experimental data, please download our application note.

Our particle-by-particle measurement greatly exceeds conventional techniques such as UV-Vis, especially as all measurements are done with no user interaction whilst the particles are offloading.

Our technology has also shown high-resolution size measurements (down to 5 nm) and can provide single nanoparticle sizing data similar to that obtained with TEM, but much faster and within native solutions, at a fraction of the TEM cost or complexity and with far less user training requirement.

In addition to mesoporous silica we are now working on characterising other drug loaded nanoparticles such as liposomes, core-shell nanoparticles, polymeric particles, metallic particles and viral vectors.

Laboratory demonstration instruments are currently available to perform proof-of-capability measurements on customer samples and our first production version of the nanoparticle analyser for nanomedicine applications is entering it’s final development stage and will be shipping in 2021.

If you would like to receive updates including copies of our application and technical notes or are interested in having samples measured. please complete our contact request form.

 

Drug unloading from mesoporous silica nanoparticles

Line graph showing decrease in drug loading (in μg/mg) over time, with a steady decrease from time 0 until 40 minutes. Shadowed regions represent 15th to 85th percentile confidence intervals.

Nanoparticle Analyser

Optical Nanoparticle Analyser (concept drawing)

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